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Test Description:
The G(-6)A angiotensinogen mutation is a point mutation found in the promoter region of the AGT gene. The mutation increases AGT gene transcription, resulting in elevated aldosterone levels. The G(-6)A mutation is associated with an increased risk for essential hypertension, thereby increasing the risk for coronary artery disease, heart failure, preeclampsia, kidney failure, stroke, and pulmonary embolism. |
Methods:
DNA is isolated from the sample submitted and tested for the G(-6)A promoter mutation in the AGT gene. The promoter region of the AGT gene is amplified enzymatically then digested with the BstN1 restriction enzyme. After digestion, the fragments are separated by electrophoresis. |
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Specimen Requirements:
5-7 ml whole blood in EDTA (purple top) tube sent with completed requisition form and billing information. Cheek brush sample using materials supplied by our laboratory is also acceptable. Copy of signed consent form should accompany specimens for carrier screening. |
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Shipping Instructions:
Send unprocessed whole blood overnight at room temperature with completed paperwork. Shipping address: MSU DNA Diagnostic Laboratory, B123 Life Sciences Bldg., East Lansing, MI 48824. Call (517)353-2032 or (877)TEST-DNA with questions. |
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Turnaround Time: 7-10 days |
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Test CPT Code: 83890, 83892, 83894, 83898, 83912 |